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The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo™ Dual-Luciferase Reporter (NanoDLR) Assay System.
The Nano-Glo™ Dual-Luciferase Reporter (NanoDLR) Assay System is a homogeneous reagent system that allows you to sequentially detect the activities of firefly (Photinus pyralis) luciferase and NanoLuc luciferase (Nluc) from a single sample.
The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.
Generate N-Terminal Fusions with NanoLuc™ Enzyme. The pNLF1 Vectors use traditional cloning with a multiple cloning site (MCS) to generate N-terminal fusions to NanoLuc™ luciferase. All vectors contain a mammalian selectable marker to create a stable line.
Generate N-Terminal fusions to NanoLuc™ Luciferase Reporter. The pFN31A and pFN31K Nluc CMV-neo Flexi™ Vectors use a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, and contain a mammalian selectable marker to create a stable line.
NanoLuc (Nluc) luciferase is a small bright luminescent reporter enzyme (19.1kDa) engineered for optimal performance. Use the pNL3.2.CMV Vector as a negative control in experiments measuring regulated changes in NanoLuc luciferase expression.
The promoter-driven NanoLuc (Nluc) control vectors can be used to co-transfect with experimental firefly luciferase vectors when using the Nano-Glo™ Dual-Luciferase Reporter (NanoDLR) Assay System.
Generate N-Terminal Fusions with N-Terminal Secreted NanoLuc™ Luciferase. The pNLF1 Vectors use traditional cloning with a multiple cloning site to generate N-terminal fusions to NanoLuc™ luciferase. All vectors contain a mammalian selectable marker to create a stable line.
The pNLCoI Vectors comprise a second-generation coincidence reporter vector system that allow expression of both firefly luciferase (luc2) and NanoLuc Luciferase fused to a PEST destabilization domain (NlucP) from the same mRNA transcript.
The Nano-Glo In-Gel Detection System directly detects the luminescence of NanoLuc fusion proteins in polyacrylamide gels after protein separation by PAGE under native or denaturing SDS conditions (native PAGE or SDS-PAGE).